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2 PhD's Biosensor for continuous monitoring with single-molecule res

Research / Academic
Eindhoven

Description:

Eindhoven University of Technology

TU/e is a University of Technology with a focus on Health, Energy and Mobility. Within the Health area, several departments cooperate on topics such as Chemical Biology, Regenerative Medicine, Computational Biology, and Biosensing, with close links to healthcare and industry. TU/e is an open and inclusive university with short communication lines. The people are curious, collaborative, and strive for excellence. TU/e enables its academic staff to develop research and education at an internationally renowned level. Our lively campus community facilitates connections between staff and students, in an open, friendly, vibrant atmosphere that welcomes and inspires.

Biomedical Engineering

The Department of Biomedical Engineering (BME) offers a research driven BME Bachelor program and Masters in Biomedical Engineering and Medical Engineering in its Graduate Program. Its research areas range from Molecular Bioengineering and Imaging, Biomechanics and Tissue Engineering to Biomedical Imaging and Modelling. The department has more than 800 students and up to 200 tenured and non-tenured employees.

Context

The group Molecular Biosensing for Medical Diagnostics (MBx, see www.tue.nl/mbx) at Eindhoven University of Technology (TU/e) creates ideas and generates scientific insights in the area of molecular biosensing with single-molecule resolution. This involves the engineering of molecular constructs for sensing applications (using protein, nucleic-acid, and protein-DNA technologies), methods to biofunctionalize nanoparticles, the development of novel single-molecule measurement methods, optical microscopies, particle characterization techniques, plasmonic effects, particle actuation principles, signal processing, and simulations. The research leads to novel methods for molecular biosensing, insights into protein function within complex and crowded environments, and novel routes into point-of-care and patient monitoring applications. The MBx group initiated and organizes the international competition on Sensors for Health (www.sensus.org). Laboratories are shared with the Chemical Biology research group (www.tue.nl/cb) and the Institute for Complex Molecular Systems (www.tue.nl/icms).  

PhD projects

The two PhD projects focus on the development of a new technology for continuous biochemical monitoring. The technology is based on the tracking of the motion of particles that are molecularly tethered to a substrate, where modulations of the motion indicate the presence of single biomarker molecules, see https://www.nature.com/articles/s41467-018-04802-8. You will investigate the feasibility to accurately monitor biomarkers by a sandwich binding format and by a competitive binding format. You will develop tools to model and analyze data from hundreds to thousands of particles at the same time. The assays will be studied in buffer and in blood plasma filtrates. Important application areas in healthcare are intensive care medicine, therapeutic drug monitoring, peri-operative monitoring, and chronic organ disease. The PhD positions are part of a collaboration between the MBx group, the Chemical Biology and Protein Engineering group, the Theory of Polymers and Soft Matter group, Leiden University Medical Center, and three companies: Medtronic, Future Diagnostics, and Anteryon.

Requirements:

We are looking for enthusiastic candidates who are in an MSc program or who already have an MSc degree, preferably in Chemistry, Physics, Biomedical Engineering, or Nanotechnology. An interest in Physical Chemistry or Biophysics is required. Experience in advanced microscopies or single-molecule methods is a plus.

Salary Benefits:

We offer:
  • A challenging job at a dynamic and ambitious University and in an enthusiastic team.
  • An appointment (1 fte) as a PhD-student for four years, starting date: flexible, but preferably as soon as possible.
  • Gross monthly salaries are in accordance with the Collective Labour Agreement of the Dutch Universities (CAO NU), increasing from € 2222 per month initially, to € 2840 in the fourth year.
  • An attractive package of fringe benefits, including excellent work facilities, end of the year allowance and sports facilities.
  • Work Hours:

    38 hours per week

    Address:

    De Rondom 70